COURSE UNIT TITLE

: RESEARCH METHODS OF CELLULAR MOLECULAR MECHANISMS

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIO 5032 RESEARCH METHODS OF CELLULAR MOLECULAR MECHANISMS ELECTIVE 2 4 0 12

Offered By

Biochemistry

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR AYŞE SEMRA KOÇTÜRK

Offered to

Audiology
Biochemistry
Laboratory Animal Science
Histology and Embryology
Anatomy
Basic Oncology
Toxicology
Molecular Medicine
Exercise Physiology
Biochemistry
Biomechanics
Biophysics
Medical Biology and Genetics
Medical Parasitology
Physiology
Medical Parasitology
Microbiology
Pharmacology
Basic Neuroscience
Molecular Medicine

Course Objective

The aim of this course is the acquisition of knowledge and experience on technics that used in cellular molecular mechanisms research. For this purpose lectures cover the principle of technics that used for isolation of organelles form tissue and cell, the principles of cell culture and application technics and analysis the expression of proteins and genes. Course programmer and applications design in a stream of all technics that provide to monitor intracellular protein and gene expressions with a tissue, a cell line and a primer cell line samples.

Learning Outcomes of the Course Unit

1   Be able to comprehend and understand of the basic cell biology
2   Be able to have a basic laboratory knowledge that can be use in intracellular molecular mechanism researches.
3   Be able to reach information and data-base related with the application area.(
4   Be able to plan a research with learned knowledge
5   Learner has a basic laboratory knowledge that can use in cellular and molecular mechanism researches

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Course presentation Application: Tissue preparation from rats. (liver and aorta)
2 Cell structure and organization, organelles, separation of organelles from a cell Application :Mitochodria isolation from liver.
3 Principles of cell culture studies (3h lecture) Application : Design and laboratory equipment and in a cell culture lab.
4 Basic rules on sterilization Application: Sterilization technics
5 The importance of cell culture techniques in research models Application: Experimental models
6 Cell viability and freezing techniques Application: Cell viability tests
7 Primer cell culture Application: To obtain a primer cell culture from rat aorta via mechanic and explant techniques
8 Analysis of protein expression-I Application: ELISA,IHK,EIA
9 Analysis of protein expression-I Application: WB
10 Analysis of Gene Expression-I Application: PCR
11 Analysis of Gene Expression-II Application: RNA isolation, RT-PCR
12 Cell Death Mechanism-I Application: Caspase enzyme activity
13 Cell Death Mechanism-II Application: Analysis of Apoptotic/ necrotic cell death
14 FINAL EXAM

Recomended or Required Reading

1. Current Protocols in Protein Science. Coligen JE, Dunn BM, Speicher W, Wingfield PT (eds). 2007, John Wiley &Sons Inc.
2. Mitochondria: A Practical Approach. Darley-Usmar UM, Rickwood D&Wilson MT (eds). 1987, IRL Pres.
3. Protein Purification Protocols. 2nd Ed. Cutler P. 2007, Humana Pres.
4. Apoptosis methods and Protocols. Brady HJM (ed) 2005, Humana Pres.
5. Apoptosis, Senescence and Cancer. Gewirtz DA, Halt SE, Grant S. (eds) 2nd ed. 2007, Humana
Press.
6. Cell and Tıssue Culture. Doyle A and Griffiths JB (eds). John Wiley&Sons.
7. Basic Cell Culture Protocols. 3rd Ed. Helgason CH, Miller CL (eds). 2004, Humana Press.
8. Real-Time PCR. Dorak TM (ed), 2007, Taylor&Francis.
9. Basic Methods fort he Biochemical Laboratory. Holtzhouer M. (ed). 2006, Springer.

Planned Learning Activities and Teaching Methods

Laboratory technics will learn with lectures and applications. Experiment design and application skills of learner will evaluate and improve via an assignment.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.40 +ASG * 0.20 + FIN* 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.40 +ASG * 0.20 + RST* 0.40

Further Notes About Assessment Methods

None

Assessment Criteria

1. Learner theoretical knowledge about research methods for molecular mechanism will evaluated with mid-term and final exam (PO1,PO2)
2. Learner skills on to reach information and data-base related with the application area, planning of experiment will evaluate via an assignment(PO6,PO7)
3. Learner knowledge on basic laboratory technics that will use to research molecular mechanism will evaluate via all application sessions and an assignment (PO10)

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

semra.kocturk@deu.edu.tr, 4124407

Office Hours

on Tuesday at: 13.30-16.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 2 30
Tutorials 15 4 60
Preparations before/after weekly lectures 7 2 14
Preparation for midterm exam 7 3 21
Preparation for final exam 7 3 21
Preparing assignments 3 50 150
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 300

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10
LO.13333333333
LO.23333333333
LO.33333333333
LO.43333333333
LO.53333333333

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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