COURSE UNIT TITLE

: SEROLOGICAL DIAGNOSIS OF PARASITIC DISEASES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ERS 6031 SEROLOGICAL DIAGNOSIS OF PARASITIC DISEASES ELECTIVE 2 2 0 9

Offered By

Medical Parasitology

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR ÜMIT AKSOY

Offered to

Medical Parasitology

Course Objective

Special immunology, immunological response to parasitic disease, defining and practising immunological methods in parasitology

Learning Outcomes of the Course Unit

1   Presents, discuss and apply knowledge about in vivo animal experiments used in Parasitology
2   Practice parasite inoculation to mice and preparing crude antigen, interpret the results
3   Practice preparing soluble antigen and interpret the results
4   Apply and compare serological tests (IHA, IFAT, ELISA, SDS-Page, Western Blot), interpret the results
5   Apply molecular tests and interpret the results

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Laboratory equipment in serology
2 Preparing buffer solutions, crude and soluble antigens in serological tests
3 Monoclonal antibody preparation and usage in parasitology
4 Antigen purification methods and usage in parasitology
5 ELISA tests (Conventional, Double Sandwich , Capture, Dot blot, Dimential) and usage in parasitology
6 Direct and indirect fluoresance antibody methods and usage in parasitology
7 Aglutination ((HA, IHA, latex, differentitial aglutination) and rapid diagnostic tests and usage in parasitology
8 SDS page, WB and usage in parasitology
9 Other serological tests (dye, avidity, compleman fixation, electrophoresis, counter immunoelectrophoresis, immunodiffusion, radioimmunoassay, competetive inhibition) and usage in parasitology
10 Diagnosis in Toxoplasmosis and interpretations of serological results
11 Diagnosis in Leishmaniosis and interpretations of serological results
12 Diagnosis in Cystic hidatidosis and interpretations of serological results
13 Diagnosis in Trichinellosis and interpretations of serological results
14 Diagnosis in Toxocariosis and interpretations of serological results
15 Diagnosis in Fascioliasis and interpretations of serological results

Recomended or Required Reading

Textbook(s): Ozcel's Medical Parasite Disease Ed. M. Ali Özcel, 2007
Others
1. Diagnostic Medical Parasitology, Ed. Lynne Shore Garcia, 2007
2. Markell And Voge's Medical Parasitology, Ed. William A. Petri, David T. John, 2006
3. Immunoresponses in parasitic infections ; Edt; EJL Soulisby , 1997
4. Basic and clinical immunology, Daniel P Stites, Appleton, Lange, 1999

Materials: For the practise, it will be used technical devices and materials in serological laboratories.

Planned Learning Activities and Teaching Methods

Learning theoretical lesson and tasks, discussion lectures, preparation presentation

Assessment Methods

Successful / Unsuccessful

Further Notes About Assessment Methods

None

Assessment Criteria

Semester exam will be in classical format and evaluated to 100 points.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

90 00 232 412 45 44

Office Hours

Tuesday , 13.30-14.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 30 1 30
Tutorials 30 2 60
Preparations before/after weekly lectures 15 1 15
Preparation for midterm exam 1 20 20
Preparing assignments 1 15 15
Reading 2 7 14
Preparation for final exam 1 40 40
Preparation for tutorial exam 1 25 25
Final 1 3 3
Midterm 1 3 3
TOTAL WORKLOAD (hours) 225

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15
LO.1555
LO.2444
LO.3444
LO.4444
LO.5444

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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