COURSE UNIT TITLE

: ELECTROPHORESIS TECHNIQUES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BIO 6061 ELECTROPHORESIS TECHNIQUES ELECTIVE 1 2 0 6

Offered By

Biochemistry

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR PINAR AKAN

Offered to

Audiology
Laboratory Animal Science
Histology and Embryology
Anatomy
Basic Oncology
Toxicology
Biochemistry
Biomechanics
Biophysics
Medical Biology and Genetics
Physiology
Medical Parasitology
Microbiology
Pharmacology
Basic Neuroscience
Molecular Medicine

Course Objective

The aim of this course is to learn the techniques of electrophoresis principal and evaluation .
In the practical related to the course, the following applications will be conducted: Serum, urine and CSF protein electrophoresis ; zimography

Learning Outcomes of the Course Unit

1   Understand, at the introductory level of electrophoresis
2   Have expertise in the theoretical basis of advance laboratory techniques
3   Have the knowledge electrophoresis medical test and interpretation the test s results
4   Present electrophorese characteristics including gel structure, voltage and their clinical aspects.
5   Demonstrate skills in laboratory work related to electrophoresis
6   Interprate serum protein electrophoresis diagrams

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 a. Introduction to electrophoresis b. Buffer systems c. Preparing different buffer systems d. Western Blotting e. 2D Electrophoresis f. Techniques to prepare agorose gel and it s staining
2 a. Serum protein electrophoresis b. CSF, urine protein electrophoresis c. Interpretation of test results d. Isoenzym electrophoresis (ALP) e. Immunfixation electrophoresis f. Zimography g. Capiller gel electrophoresis h. isoelectric focusing

Recomended or Required Reading

1.Electrophoresis in practice, A guide to methods and applications of DNA and protein seperationThird Edition.,Eds: Sonja Gronau,PhilBeckett,2004.
2. Gel electrophoresis of proteins, Third Edition,Eds: B.D. Hames,1998.
3. Clinical Chemistry, Third Edition., Eds:.Cari A. Burtis, Edward R. Ashwood, 1996.
4. McPherson: Henry's Clinical Diagnosis and Management by Laboratory Methods, 22nd ed.2011 Saunders, An Imprint of Elsevier , Chapter 19.
5. Inman Z. et al. Reporting of Quantitative Protein Electrophoresis in Australia and New Zealand: a Call for Standardisation Clin Biochem Rev 2009; 30 : 141
6. Electrophoressis Atlas of Serum proteins, Serum Immunfixation, Urine Proteins and Cryoglobulins, A. Ciapini,Interlab.

Planned Learning Activities and Teaching Methods

The course will be run in an integrated way-winding theory with case discussion and laboratory practicals. The methods of learning will be interactive.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 PRF PERFORMANCE
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE PO * 0.40 + FN*0.60
4 RST RESIT
5 FCGR FINAL COURSE GRADE (RESIT) PO * 0.40 + BUT*0.60

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

The student is expected to read the resources before coming to class. In addition, he/she is expected to contribute actively to discussion, group-work and practicals. Attending in at least 70 percent of lectures and in at least 80 percent of practicals are mandatory. Plagiarism of any type will result in disciplinary action.

Contact Details for the Lecturer(s)

Pınar Akan:
Phone: 412 44 11/ 25 52
GSM: + 536 341 51 30

Office Hours

Every day, 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 2 26
Tutorials 20 2 40
Preparations before/after weekly lectures 13 1 13
Preparation for final exam 1 20 20
Preparing assignments 1 19 19
Preparing presentations 1 10 10
Practice in Legal Institutions 19 1 19
TOTAL WORKLOAD (hours) 147

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14
LO.133344333333333
LO.233344333333333
LO.333344333333333
LO.433344333333333
LO.533344333333333
LO.633344333333333

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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