COURSE UNIT TITLE

: ADVANCED CANCER CELL CULTURE AND APLLICATIONS

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ONK 6085 ADVANCED CANCER CELL CULTURE AND APLLICATIONS ELECTIVE 1 2 0 6

Offered By

Basic Oncology

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR ZEKIYE SULTAN ALTUN

Offered to

Basic Oncology

Course Objective

The aim of this course the acquisition of basic knowledge about cancer research specific cell cultures which is one of the techniques commonly used techniques associated with it
and to introduce the necessary tools to make cell culture and related laboratory methods and thus their thesis and research projects associated with cancer cell culture studies will continue.

Learning Outcomes of the Course Unit

1   Capable of outlined in the culture of cancer cells can express (knowledge).
2   Be able to apply the basic methods of Cancer cell culture (Application)
3   Be able to recognize and understand the types of cancer cells
4   Be able to identify of Cancer cell viability, and interpret (evaluation)
5   Be able to research and inquire about the work of cancer cell culture (analyses)

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Basic cell culture laboratory layout
2 The cell culture of the materials used and their preparations
3 Techniques of sterilization
4 Cell culture media
5 Contamination and prophylactic use of antibiotics in cells and tissues
6 Cancer-specific methods used in the selection of cells
7 Haemocytometer; cell counting, viability cytotoxicity
8 presentations of homework
9 Cancer-specific identification tests
10 Passage techniques
11 Primary and secondary cell culture
12 Techniques of Storing, living and freezing of cells : criyoprezervation-1
13 The characterization of cancer cell lines
14 Cancer cell culture-1
15 Cancer cell culture-2

Recomended or Required Reading

Prof.Dr. Ahmet Münir KINAY,Prof.Dr. Ahmet Uğur YILMAZ,Prof. Dr. Hülya ELIDOKUZ,Prof.Dr. Safiye AKTAŞ,Doç.Dr. Zekiye Sultan ALTUN,Doç Dr. Yasemin Baskin,MSc. Pınar Erçetin,YL Öğr Gizem Çalıbaşı,"Research methods used in basic ", Dokuz Eylül Üniversitesi, Izmir, (2011) ,Handbook.
Supplementary Book(s): Cancer Cell Cultures Methods and Protocols, Simon P. Langdon, Humana Press.
References: Zekiye Sultan Altun, Serpil Tanrıverdi Akhisaroğlu, Janserey Batu, Halil Ateş, Hatice Şimşek, Semra Koçtürk,"Discrimination effectiveness of CK18 on cell death modes in colon cancer cells", Turkish Journal of Biochemistry, 35(1):20-28, 2010. Zekiye Sultan Altun, Dilek Güneş, Safiye Aktaş, et al.,"Protective Effects
of Acetyl-L-Carnitine on Cisplatine Cytotoxicity and Oxidative Stress in Neuroblastoma.",
Neurochemical Research, 35(3):437-43, 2010

Planned Learning Activities and Teaching Methods

Theoretical and applied Lectures, cancer cell culture practices, transactions related to the cell culture itself by given a cell to students to practice with it, presentation about the culture of cancer cells by students, giving a homework and having a final exam.
Students will prepare homework related to primary cancer cell cultures of lung cancer, breast and colon cancer.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 PRS PRESENTATION
3 FCG FINAL COURSE GRADE
4 FCG FINAL COURSE GRADE ODV * 0.30 + PRJ * 0.20 + FN* 0.50
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ODV * 0.30 + PRJ * 0.20 + BUT* 0.50

Further Notes About Assessment Methods

None

Assessment Criteria

The learner will clearly define outlined cancer cell culture which will evaluate with presentations prepared by themselves, homework and their laboratory applications .
2. The learner will clearly apply basic methods of cancer cell culture laboratory practices.

3. The learner will assignments related to the cancer cell types, making the presentation and homework.
4. The learner will clearly identify and interpret of cancer cell viability with laboratory practices
5. The learner will recognize cancer cell culture studies; will evaluate with homework, prepared presentations by themselves and exam.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

zekiye.altun@deu.edu.tr 0 232 4128204/5801

Office Hours

on Tuesday at: 13.30-16.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 1 15
Tutorials 14 2 28
Preparations before/after weekly lectures 14 2 28
Preparing assignments 1 20 20
Lab Preparation 14 2 28
Preparation for final exam 1 28 28
Final 1 3 3
TOTAL WORKLOAD (hours) 150

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.155555555
LO.2555555
LO.35555555
LO.4555555555
LO.55555555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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