COURSE UNIT TITLE

: LABORATORY ROTATION IN MOLECULAR MEDICINE I

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
MOT 5007 LABORATORY ROTATION IN MOLECULAR MEDICINE I COMPULSORY 1 4 0 8

Offered By

Molecular Medicine

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR GÜL HÜRAY IŞLEKEL

Offered to

Molecular Medicine

Course Objective

The aim of this course is to allow the students to rotate in different laboratories
related with the MSc programme in molecular medicine in a multidisciplinary
perpective, to learn about the different research methods and techniques used in
research laboratory and to acquire practical knowledge in this area. Basic methods
learnrd are as follows: LC-MS/MS, gene clonning, transformation, transfection, siRNA,
plazmid izolation, cell culture, Gdna, RNA, protein (total, nuclear) izolation, Agaroz
gel electrofhoresis, SDS-PAGE gel electrofhoresis, RT-PCR, real time PCR, western
blotting, ımmunopresipitation, ELISA, immunofloresan staining, histone izolation,
microarray anaysis, bioinformatics (GEO, GO, ALIGN, EntrezGene data base), cell biology
methods

Learning Outcomes of the Course Unit

1   To know about the different laboratories which may be involved in molecular medicine
2   To learn about the different subjects and areas of application in these laboratiores
3   To understand the basic principles of techniques used in these laboratories
4   To compare different methods in terms of principles and applications
5   To be able to apply these methods in ones own reseasrch field
6   To integrate the information acquired and prepare an oral presentation

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Spektrofotometry, Elektrophoresis, cell culture practice
2 Western Blotting, PCR, atomik absorption
3 Spektroflourometry, flourometric free radical measurement
4 Chromatography LC/MS-MS practices
5 TEM Basic principals and practices
6 TEM Basic principals and practices
7 Işık mikroskobunda değerlendirme
8 TEM staining, TEM Image Processing
9 Observation and practices of Molecular biology techniques used in Molecular biology laboratories
10 Method evaluation 1
11 Method evaluation 1
12 Method evaluation 1
13 Method evaluation 1
14 Evaluation of Laboratory Methods

Recomended or Required Reading

1. Biomedical Science Practice-Experimentla and Professional skills (Edited by Hedley
Glencross, Nessar Ahmed and Qiuyu Wang). Oxford University press. 2011
2. Tietz Klinik Kimyada Teme Ilkeler (Beşinci Baskıdan Çeviri) Editörler: Catl A.
Burtis- Edward R. Ashwood Çeviri Editörü: Diler Aslan. Palme Yayıncılık. 2005
3. Articles on laboratory methods published in SCI or expanded SCI journals.

Planned Learning Activities and Teaching Methods

The course will be organised as rotations in different labs. There will be short
presentations from the instructors on the different methods. The students will take
notes and prepare a laboratory notebook involving the information learned and
processed. In addition, they will be expected to present orally what they have learned.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 PRF PERFORMANCE
3 FIN FINAL EXAM
4 FCGR FINAL COURSE GRADE MTE * 0.30 + PRF* 0.30 + FIN* 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + PRF* 0.30 + RST* 0.40

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

The student is expected to read the resources before coming to class. In addition,
he/she is expected to contribute actively to discussion.

Contact Details for the Lecturer(s)

Prof Dr. HÜRAY iŞLEKEL
Phone: 412 44 08

Office Hours

Tuesday: 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 4 56
Preparations before/after weekly lectures 14 3 42
Preparation for midterm exam 2 10 20
Preparation for final exam 1 30 30
Preparing presentations 2 10 20
Preparing the laboratory notebook 2 16 32
TOTAL WORKLOAD (hours) 200

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14
LO.144553343343335
LO.244555555554555
LO.344554444444555
LO.455555554544555
LO.555555555544555
LO.655555555555445

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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