COURSE UNIT TITLE

: BIOCHEMISTRY LABORATORY II

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 3016 BIOCHEMISTRY LABORATORY II COMPULSORY 0 3 0 3

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR DOCTOR LEMAN TARHAN

Offered to

Chemistry
Chemistry (Evening)

Course Objective

In this course; the aim is to learn basic techniques to students in biochemistry laboratory.

Learning Outcomes of the Course Unit

1   Analyze quantitatively glucose, pyruvate, vitamin C and storage polysaccharide determination in different samples and evaluate the results.
2   Perform enzymatic synthesis and characterization of the Glucose-1-phosphate from starch and discuss the results.
3   Plan experimental steps required for enzymatic synthesis of linear starch, apply and evaluate the results.
4   Plan experimental steps required for cholesterol determination, apply and evaluate the results.
5   Determine lipid peroxidation and evaluate the results.
6   Plan experimental steps to determine urea in urine, apply and discuss the results.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 GENERAL PRINCIPLES IN BIOCHEMISTRY LABORATORY, CHEMICAL HYGENE, STATISTICAL ANALYSIS
2 DETERMINATION OF PYRUVATE AND ASCORBIC ACID
3 ENZYMATIC SYNTHESIS OF GLUCOSE-1-PHOSPHATE FROM STARCH-I
4 ENZYMATIC SYNTHESIS OF GLUCOSE-1-PHOSPHATE FROM STARCH-II
5 ENZYMATIC SYNTHESIS OF GLUCOSE-1-PHOSPHATE FROM STARCH-III
6 LIPID PEROXIDATION
7 CHOLESTEROL DETERMINATION
8 Mid-term exam
9 SPECTROPHOTOMETRIC DETERMINATION OF PROLIN IN PLANTS
10 UREA AND INORGANIC PHOSPHATE DETERMINATION
11 MICROBIAL PROTEIN PRODUCTION VIA FERMANTATION TECHNOLOGY AND PURIFICATION-I
12 MICROBIAL PROTEIN PRODUCTION VIA FERMANTATION TECHNOLOGY AND PURIFICATION-II
13 EXPERIMENTAL DESIGN
14 Compensation

Recomended or Required Reading

Textbook(s): Bonner, P.L.R., Hargreavees, A.J., Basic Bioscience Laboratory Techniques, Willey-Blackwell, 2010.
Supplementary Book(s): Glencross H, Ahmed N, Wang Q, Biomedical Science Practice, 978-0-19-953329-9, Oxford University Press.

Planned Learning Activities and Teaching Methods

Interactive expression, Question and answer technique, Experimental application and reporting

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.30 + ASG * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.30 + RST * 0.40


*** Resit Exam is Not Administered in Institutions Where Resit is not Applicable.

Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, reports. The lecturer informs the student about the assignments and their collection dates within first four weeks. The assignments with signatures are collected within the last two weeks.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Prof. Dr. Leman TARHAN
Dokuz Eylul University, Faculty of Science, Department of Chemistry
e-mail: leman.tarhan@deu.edu.tr

Office Hours

Wednesday 15.00-17.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Tutorials 13 4 52
Preparation before/after weekly lectures 10 1 10
Preparation for Mid-term Exam 1 2 2
Preparation for Final Exam 1 3 3
Preparing Individual Assignments 10 1 10
Final 1 2 2
Mid-term 1 2 2
TOTAL WORKLOAD (hours) 81

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11PO.12PO.13PO.14PO.15PO.16PO.17
LO.12555345555
LO.225553345555
LO.335553345555
LO.43555345555
LO.51555345555
LO.63555345555