COURSE UNIT TITLE

: BIOCHEMISTRY LABORATORY I

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 3025 BIOCHEMISTRY LABORATORY I COMPULSORY 0 3 0 3

Offered By

Chemistry

Level of Course Unit

First Cycle Programmes (Bachelor's Degree)

Course Coordinator

PROFESSOR RAZIYE ÖZTÜRK ÜREK

Offered to

Chemistry
Chemistry (Evening)

Course Objective

In this lecture the students will learn the basic techniques used in biochemistry laboratory.

Learning Outcomes of the Course Unit

1   Make experimental planning and applications in order to reveal some properties (colorization reactions, amphoteric character etc.) of protein and amino acids, and evaluate the results.
2   Isolate protein and determine the protein contents in biological sources by using spectrophotometric method and evaluate the results.
3   Determine enzyme activity spectrophotometrically and evaluate the results.
4   Examine the effects of factors such that amount of enzyme and substrate, pH and temperature on the enzyme activity and evaluate the results.
5   Indicate presence of carbohydrate in biological sources and osazone formation and experimentally assay of glucose, evaluation of the results.
6   Determine experimentally number of saponification and solubility of lipids and evaluate the results.
7   Isolate DNA from biological source and evaluate the results.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Rules to be obeyed and basic techniques used in biochemistry laboratory
2 Determination of some properties of protein and amino acids ( colorization reactions and amphoteric character)
3 Determination of amino asid pI value and quantitative analysis by spectrophotometric method
4 Quantitative analysis of protein from biological resourse by spectrophotometric methods
5 Isolation of protein from various biological sources
6 Applications of enzyme activity assays by different methods
7 Substrate and enzyme-dependent change in the amount of enzyme activity
8 Midterm Exam
9 pH and temperature-dependent change in enzyme activity
10 Kinetic of enzyme inhibition
11 Determination of the presence of carbohydrate in various samples and the formation of osazone
12 Investigation of the solubility of lipids and determination of number of saponification
13 Isolation of DNA
14 Compensation

Recomended or Required Reading

Textbook(s): Bonner, P.L.R., Hargreavees, A.J., Basic Bioscience Laboratory Techniques, Willey-Blackwell, 2010.
Supplementary Book(s): Glencross H, Ahmed N, Wang Q, Biomedical Science Practice, 978-0-19-953329-9, Oxford University Press.

Planned Learning Activities and Teaching Methods

Interactive expression, Questions and answers, Practice in laboratory

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PAR PARTICIPATION
4 FIN FINAL EXAM
5 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.30 + ASG * 0.20 + PAR * 0.10 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the concepts of given experimental applications, association to concepts each other, establish cause-effect relationships, to using existed knowledge for evaluation to questions or problems and evaluating of generation ideas are carried out by using the midterm exam, final exam, active participation in the experimental practice.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry
e-mail: raziye.urek@deu.edu.tr, hulya.kayali@deu.edu.tr

Office Hours

Wednesday 15.00-17.00

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Tutorials 13 3 39
Preparation for Mid-term Exam 1 2 2
Preparation for Final Exam 1 5 5
Preparing Individual Assignments 11 1 11
Preparation before/after weekly lectures 13 0,5 7
Final 1 2 2
Mid-term 1 2 2
TOTAL WORKLOAD (hours) 68

Contribution of Learning Outcomes to Programme Outcomes

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LO.2555555555
LO.3555555555
LO.455555555
LO.555555555
LO.655555555
LO.7555555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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