COURSE UNIT TITLE

: ADVANCES IN GAS CHROMATOGRAPHY AND HPLC

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 5022 ADVANCES IN GAS CHROMATOGRAPHY AND HPLC ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR MEHMET KADIR YURDAKOÇ

Offered to

Chemistry
Chemistry

Course Objective

Introduction and principles of gas chromatography and HPLC will be explained in the first chapter. Techniques and instrumentation will be discussed in detail. Some applications of GC and HPLC will also be emphesized in detail. HPLC detectors, packingsand hardware will be explained. Size exclusion chromatography, reversed phase chromatography and related techniques will also be given in this lecture.

Learning Outcomes of the Course Unit

1   After this course, the students will be able to explain the fundamental concepts & theories of separation techniques in GC and HPLC.
2   to sketch and label the schematic diagrams, to discuss the function of each component in GC, HPLC and GC-MS instruments.
3   to identify the strength & limitations of GC, GC-MS and HPLC techniques
4   to learn about the developments in the area of GC, GC-MS and HPLC
5   to suggest and justify the most suitable, correct , efficient and sufficient separation technique to be employed for an analysis among these methods.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction, Theory and Basics of GC: Theory of Gas Chromatography, Columns: Packed and Capillary/Column Selection in Gas Chromatography
2 Optimizing Separations in Gas Chromatography, Presentation of References and Literature about this Lecture
3 Techniques and Instrumentation: Detectors and Data Handling, Techniques for Gas Chromatography/ Mass Spectrometry, Qalitative and quantitative Analysis by Gas Chromatography: Sample Preparation and Trace Analysis
4 Applications: Physicochemical Measurements by Gas Chromatography; Petroleum and Petrochemical Analysis by Gas Chromatography; Polymer Analysis Using Gas Chromatography; Clinical Applications of Gas Chromatography 3.5. Environmental Applications of Gas Chromatography
5 Evaluation the GC data, possible error sources and related solutions of them
6 GC-MS techniques and instrumentations
7 Midterm
8 HPLC: Theory, Hardware, Detectors, Packings of HPLC; Size Exclusion Chromatography; Reversed-Phase Chromatography; Ion-Exchange Chromatography; Capillary Electrophoresis
9 Size Exclusion Chromatography; Reversed-Phase Chromatography; Ion-Exchange Chromatography; Capillary Electrophoresis
10 Sample Preparation, Quantitation and Data Handling in HPLC and related techniques
11 Troubleshooting, Method development and validation in these techniques
12 Further reading, Applications and literature survey in HPLC and GC-MS
13 General Evaluation and Make-up and discussions on recent articles in GC and HPLC
14 Homework discussions and presentations

Recomended or Required Reading

1. Gerhard Schomburg, Gas Chromatography, VCH Verlag, Weinheim, 1990.
2. Robert L. Grob, Modern Practice of Gas Chromatography, John Wiley&Sons, 1995.
3. Robert L. Cunico, Karen M. Gooding, Tim Wehr,Basic HPLC and CE of Biomolecules, Bay Bioanalytical Laboratory, 1998.
4. Lloyd R. Snyder, Joseph L. Glajch, Joseph Kirkland, Practical HPLC Method Development, John Wiley&Sons, 2nd Edition, 1997.

Planned Learning Activities and Teaching Methods

Fourteen, three-hour lectures backed up with one midterm examination and one workshop and interactive presentations. All the didactic material is preliminary available to the student both in paper and electronic form. The books used to prepare the lectures are available at the Library of the University. Further data and/or information may be obtained from internet using the new wireless network.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40


Further Notes About Assessment Methods

Lecture, Discussion, Question & Answer, Field Trip, Team/Group Work, Demonstration, Experiments with HPLC, Drill - Practise, Case Study, Brain Storming

Assessment Criteria

Midterm(%30)+ Homework(%20)+Presentation(%10)+Final exam(%40)

Language of Instruction

English

Course Policies and Rules

Students will attend 14 lectures and 2 tutorials and 1 workshop on the material covered in this course. Attendance is compulsory at both till %70, but the rules for the attendence up to the students and can be obtained from the web site of Graduate School of Sciences at http://www.fbe.deu.edu.tr/.

Contact Details for the Lecturer(s)

Prof. Dr. Mehmet Kadir Yurdakoç
Dokuz Eylül University, Faculty of Science, Department of Chemistry
Phone: (232) 3018695
E-mail: k.yurdakoc@deu.edu.tr

Office Hours

Wednesdays 5.,6.th hours.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 14 3 42
Tutorials 2 1 2
Preparations before/after weekly lectures 14 3 42
Preparation for midterm exam 1 25 25
Preparation for final exam 1 30 30
Preparing assignments 2 15 30
Preparing presentations 1 25 25
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 200

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.122
LO.232
LO.3432323
LO.4432323
LO.5432323

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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