COURSE UNIT TITLE

: IMMOBILIZED AFFINITY LIGAND TECHNIQUES

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYT 5017 IMMOBILIZED AFFINITY LIGAND TECHNIQUES ELECTIVE 2 0 0 7

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

The immobilization of peptides, proteins, antibodies, enzymes, nucleic acids and many other affinity ligands will be exemplified by discussing the topics such as bases of affinity chromatography, immobilized affinity ligands used in different systems, how these ligands are applied, selection of matrix, methods of matrix activation, ligand immobilization, and the course will include the application areas of affinity systems depend on purpose.

Learning Outcomes of the Course Unit

1   Have knowledge about natural and synthetic carriers used in affinity ligand techniques,
2   Discuss and have knowledge about activation methods of various carriers,
3   Discuss and have knowledge about ligand immobilization methods,
4   Discuss yieldance of ligand activation,
5   Have knowledge and give example about purification, seperation, catalysis and modification, applications of affinity ligands.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Carriers Used in Affinity Ligand Techniques
2 Synthetic Carriers
3 Carrier Activation Methods-1; Activation and Ligand Binding Procedures of Amine Reactive Carriers - Activation and Ligand Binding Procedures of Sulfhydryl Reactive Carriers
4 Carrier Activation Methods -2; Activation and Ligand Binding Procedures of Carbonyl Reactive Carriers - Activation and Ligand Binding Procedures of Hydroxyl Reactive Carriers - Activation and Ligand Binding Procedures of Active Hydrogen Reactive Carriers - Photo Reactive Cross-Linkers and Photochemical Modification Procedures
5 Ligands Immobilization Methods-1; Small Ligands
6 Ligands Immobilization Methods-2; Small Ligands
7 Ligands Immobilization Methods-3; General Protein Immobilization
8 Midterm Exam
9 Ligands Immobilization Methods-4; Enzyme Immobilization Lectin Immobilization
10 Ligands Immobilization Methods-5; Peptide Antigens, Antibodies and Immunoglobulin Binding Proteins
11 Ligands Immobilization Methods-6; Nucleic Acid Immobilization - Thiophilic Adsorbents - Immobilized Disulfide Reductants
12 Analysis Methods; Measurement of Activation Levels and Immobilized Ligand Density - Affinity Techniques
13 Selected Applications; Purification, Selective Separation: Catalysis and Modification - Analytical Applications of Affinity Ligands
14 Homework and Presentations

Recomended or Required Reading

Textbook(s): Hermanson, G. T., Mallia A. K. & Smith, P. K. Immobilized Affinity Ligand Techniques Academic Press, 1992.
References: Kirschning, A. & Bannwarth, W. Immobilized Catalysts: Solid Phases, Immobilization and Applications Springer, 2004.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Question-answer technique
3. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, homework and presentations.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Science Faculty, Chemistry Department
leman.tarhan@deu.edu.tr

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 2 26
Preparation before/after weekly lectures 13 3 39
Preparing Homework and Presentations 1 30 30
Preparation for Final Exam 1 35 35
Preparation for Mid-term Exam 1 30 30
Final 1 2 2
Mid-term 1 2 2
TOTAL WORKLOAD (hours) 164

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6
LO.1545545
LO.2545545
LO.3545545
LO.4545545
LO.5555545

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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