COURSE UNIT TITLE

: RECOMBINANT TECHNIQUES IN BIOTECHNOLOGY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYT 6013 RECOMBINANT TECHNIQUES IN BIOTECHNOLOGY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

Developments in molecular biology in the last 30 years, has allowed recombinant technologies to play an important role in the progression of biotechnology techniques. In this course, there will be an introduction to DNA starting with the basic methods of work to do, and cloning, genetic engineering studies which performed by using them the will be examined in detail. It will be described that how these techniques are used in the amplification steps of proteins primarily in bacteria and later in the eukaryotic cells, these methods will be compared and current examples will be shared for each of them.

Learning Outcomes of the Course Unit

1   Students will gain general view about the expression of recombinant proteins.
2   Students will obtain information on the use of procaryotic and eukaryotic cells in protein expression .
3   Students will obtain information on the use of procaryotic and eukaryotic cells in the application of these expression systems in biotechnology.
4   Students will obtain information on PCR reactions and basic components.
5   Students will obtain information on transgenic technology; basic principles, application in animals and plants.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Overview to the recombinant biotechnology
2 Basic concepts in molecular biology 2.1. Nucleic acids, structure of DNA 2.2 Transcription and translation
3 Enzymes used in molecular biology 3.1. Restriction endonucleases
4 Polymerase chain reaction (PCR) 4.1. PCR reaction and its basic components 4.2. Enzymes used in PCR and their applications 4.3. Usage areas of PCR
5 Gene cloning 5.1. The principle of cloning 5.2. Molecular methods used for the verification of cloning
6 Protein expression in bacteria I- Fundamentals 6.1. Bacteria 6.2. Plasmids 6.3. Vectors
7 Protein expression in bacteria II- Problems 7.1. Problems in protein folding and glycosylation
8 MIDTERM EXAM
9 Fundamentals of protein expression in eukaryotic cells 9.1. Cells 9.2. Vectors
10 Transgenic technology 10.1. Basic principles 10.2. Animal and plant applications
11 11.1. Cloning of an animal: farm animals as a protein factory 11.2. Living cloning: basic principles 11.3. Examples
12 Recombinant antibody technology
13 Production of recombinant proteins in bacteria 13.1. Industrial Applications Production of recombinant proteins in eukaryotic cells 13.2 Industrial Applications 13.3 Biosimilars
14 Homework and Presentations

Recomended or Required Reading

Textbook(s): Biotechnology: Applying the Genetic Revolution D.P. Clark and N.J. Pazdernik (2008)

Planned Learning Activities and Teaching Methods

1. Presentation
2. Question-answer technique
3. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, homework and presentations.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Vocational School of Health Services
kemal.baysal@deu.edu.tr

Office Hours

Wednesday 13.30 - 15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 35 35
Preparation for final exam 1 40 40
Preparing Homework and Presentations 1 35 35
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 192

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6
LO.1544453
LO.2544453
LO.3454543
LO.4545453
LO.5545453

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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