COURSE UNIT TITLE

: PLANT BIOTECHNOLOGY

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYT 6008 PLANT BIOTECHNOLOGY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

Ph.D. in Biotechnology
BIOTECHNOLOGY

Course Objective

In this course it is anticipated to give information on the plant biotechnology. Plants expose to varies disease and harmfull weed during production process of the plant and due to this problem yield of plant go down. To eliminate the risk, modern biotechnology methods are improved.

Learning Outcomes of the Course Unit

1   Students will learn plant genoms; DNA, chromatin, structure of chromosome and gen.
2   Students will have an idea plant cell culture and types of culture.
3   Students will learn techniques of plant transformation and improving plant transformation.
4   Students will learn how to be improved transgene resistant to herbicide and insect.
5   Students will study resistance of plant diease and learn to be decreased effect of viral disease.
6   Students will learn that product quality and yield to be increased.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Plant genomes: Plant Genome Organization and Expression of Genes 1.1. DNA, Chromatin and Chromosome Structures 1.2. Introduction to gene structure and gene expression 1.3. Regulation of Gene Expression 1.4. Plant Transformation 1.5. Protein Targeting 1.6. Genome Size and Organization 1.7. Heterologous Promoters 1.8. Arabidopsis and New Technologies
2 Plant Tissue Culture 2.1. Introduction to Plant Tissue Culture 2.2. Types of Culture 2.3. Plant Regeneration 2.4. Integration of Plant Tissue Culture to Plant Transformation Protocols
3 Plant Transformation Techniques 3.1. Agrobacterium-Dependent Gene Transfer 3.2. Ti plasmid 3.3. T-DNA Transfer Process and Integration 3.4. Agrobacterium-Dependent Practical Applications of Plant Transformation 3.5. transformation of Plantas 3.6. Direct methods used to transfer gene
4 Binary vectors at plant transformation 4.1. Seeking Properties of Plasmid vectors 4.2. Evolution of Plant Transformation Vectors 4.3. Basic Features of Plant Transformation Vectors 4.4. Optimization 4.5. Gene Technology
5 Development of Herbicide-Resistant transgenicPlants 5.1. Use of herbicides in modern agriculture 5.2. Herbicides and Mechanisms of Activity 5.3. obtainment of Herbicide Resistant Transgenic Plants 5.4. Environmental Effects on Herbicide-resistant crops
6 Development of Insect-Resistant Transgenic Plants 6.1. The nature and rate products Nuisance Insects 6.2. Bacillus thuringiensis 8-endotoxins 6.3. Carrying the gene of Bt Transgenic Plants 6.4. Insect Resistant Products, and Food Safety
7 Plant Disease Resistance 7.1. Plant-Pathogen Interactions 7.2. Natural Resistance Pathways to Disease 7.3. Biotechnological resistance Approaches to disease
8 MIDTERM EXAM
9 Reducing the Impact of Viral Diseases 9.1. Types of Plant Virus 9.2. Replication and inhibition Destinations 9.3. Development of virus-resistant transgenic plants
10 Physiology of Stress Resistance in Plants 10.1. Term of Stress and Stress Factors 10.2. Abiotic Stress and Water Deficiency Stress 10.3. Secondary Effects of Abiotic Stress- Production of Reactive Oxygen Species
11 Enhancment of fertileness and Quality of Product 11.1. Genetic Manipulation at Fruit ripeness 11.2. Genetic Manipulation for increasing fertileness of product with Photosynthesis
12 Molecular Farming 12.1. Carbohydrates and Fats 12.2. Molecular Farming of Proteins 12.3. Economic Aspect in farming Molecular
13 Future Expectations of Genetically Modified (GM) Product 13.1. The current status of transgenic products 13.2. Concerns Related to GM Products 13.3. Regulation of GM Products and Products 13.4. Future Development of Plant Biotechnology
14 Homework and Presentations

Recomended or Required Reading

Textbook(s): Slater, A., Scott, N., & Fowler, M.,Plant Biotechnology The Genetic Manipulation of Plants, Oxford University Press, New York, 2003.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Question-answer technique
3. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, homework and presentations.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry
hulya.kayali@deu.edu.tr

Office Hours

Wednesday 13.30 - 15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 35 35
Preparation for final exam 1 40 40
Preparing Homework and Presentations 1 35 35
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 192

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6
LO.1544553
LO.2554444
LO.3554435
LO.4455534
LO.5544434
LO.6534434