COURSE UNIT TITLE

: TIME RESOLVED FLUORESCENCE ANALYSIS OF NANOMATERIALS

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
NNE 5010 TIME RESOLVED FLUORESCENCE ANALYSIS OF NANOMATERIALS ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR ÖZLEM ÖTER

Offered to

Nanoscience and Nanoengineering
Nanoscience and Nanoengineering

Course Objective

This course aims to gain an theoretical based understanding on the principles of time resolved fluorescence technique and fluorescence lifetime from milliseconds to picoseconds and the gaining of the knowledge of characterization, understanding, design and quantification of fluorescent materials, nanoparticles, nanofibers, quantum dots, optical sensors and nanosensors and spectral data analysis with time resolved fluorescence spectroscopy.

Learning Outcomes of the Course Unit

1   This course is expected to understand the student to gain basic knowledge on time resolved fluorescence spectroscopy (TRFS).
2   To exploit TRFS in nano technology or daily life.
3   To develop the students analytical thinking abilities to present new and creative approaches regarding the design of nanomaterials e.g. nano sensors, nanofibers, quantum dots.
4   To develop the students analytical thinking abilities to present new and creative approaches regarding the characterization of nanomaterials e.g. nano sensors, nanofibers, quantum dots.
5   To interpret the TRFS based spectral data.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Theoretical base of time resolved fluorescence technique and fluorescence lifetime
2 Time domain and frequency domain lifetime measurements from milliseconds to picoseconds.
3 Time correlated single photon counting method (TCSPC) and Single/Multiexponential decays
4 Components for Time correlated single photon counting method (Light sources, electronics, detector systems according to the sample etc.)
5 Interpretation of Time Resolved Fluorescence Spectra and data analysis
6 Applied nanofluorescence to characterize, and quantify nanomaterials
7 Quenching based time resolved fluorescence applications for the analysis of nanomaterials
8 Energy transfer based time resolved fluorescence applications for the analysis of nanomaterials
9 Mid-term exam.
10 Molecular Applications of time correlated single photon counting (E.g. Intensity decays for nanoaggregates, intensity decays for fluorescent nanoparticles, nano fibers and carbon nanotubes)
11 Analysis of nano-organic carbon (NOC) particles by means of time-resolved fluorescence polarization anisotropy
12 Multifunctional nanoparticles for time-resolved fluorescence cell imaging
13 Fluorescence lifetime based nanosensor designs and Innovations in time resolved fluorescence spectroscopy and in vivo imaging
14 Final exam

Recomended or Required Reading

Principles of Fluorescence Spectroscopy, Joseph R. Lakowicz, Kluwer Academic, New York, 1999.

Planned Learning Activities and Teaching Methods

The course will go on as class presentation and sometimes will be in interactive format. All course members are expected to attend the lectures and submission hours and take part in the submission discussion sessions. Besides, oral presentations on selected topics are to be prepared by the students.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 PRJ PROJECT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE + PRJ/2 * 0.50 +FIN * 0.50
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE + PRJ/2 * 0.50 + RST * 0.50


Further Notes About Assessment Methods

There will be minimum 2 presentations, averaged out grades for which will be 25 % of the overall success of the students. One mid-term examination will be averaged and affect the grade by 25 %. Final exam will affect 50 % of the grade.
Homework
Midterm Homework Submission (Oral presentation)
Final Exam

Assessment Criteria

There will be minimum 2 presentations, averaged out grades for which will be 25 % of the overall success of the students. One mid-term examination will be averaged and affect the grade by 25 %. Final exam will affect 50 % of the grade.
Homework
Midterm Homework Submission (Oral presentation)
Final Exam

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Assoc. Prof. Dr. Özlem Öter
ozlem.oter@deu.edu.tr
Tel: 0232 301 95 06

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 8 3 24
Presentations 6 3 18
Tutorials 14 5 70
Preparation before/after weekly lectures 14 2 28
Preparing presentations 2 10 20
Preparing group assignments 2 10 20
Preparing for midterm/final exam 1 18 18
Midterm 1 1 1
Final 1 1 1
TOTAL WORKLOAD (hours) 200

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7
LO.11525152
LO.22134251
LO.35152432
LO.44221522
LO.53113111