COURSE UNIT TITLE

: ENZYMATIC ANALYSIS METHODS

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 5107 ENZYMATIC ANALYSIS METHODS ELECTIVE 3 0 0 7

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

M.Sc. in Biochemistry
Chemistry
Ph.D in Biochemistry

Course Objective

The applicable techniques, procedures, data analysis techniques to practice enzymatic analyses in plant, animal and microorganism samples will be discussed for the researchers in the field of Biochemistry, Molecular Biology and Biology.

Learning Outcomes of the Course Unit

1   Examine the effective factors in enzyme activity measurements,
2   Have a knowledge as to the preparation of sample depend on reagent and source in enzymatic analysis,
3   Examine appropriate assay methods depend on enzymatic reaction,
4   Make evaluations for enzyme kinetic,
5   Examine the statistical differences in the enzyme activity assay.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Enzymes and Basic Principles in Enzymatic Analysis
2 Enzymatic Reaction Kinetic and Basic Parameters
3 Enzyme Cataliytic Activity Assays
4 Enzyme Active Region Assay
5 Reagent and Sample Preparation Methods in Enzymatic Analysis
6 Enzyme Activity Assays-I: Photometry, Nephelometry, Turbidimetry
7 Enzyme Activity Assays-II: Potentiometry, Conductimetry, Polarimetry
8 Midterm exam
9 Enzyme Activity Assays-III: Luminometry, Enthalpymetry, Radiometry
10 Enzyme Activity Assays-IV: High Performance Liquid Chromatography Analyses
11 Enzyme Activity Assays-V: Radiometry
12 Enzyme Activity Assays-VI: Gel electrophoresis
13 Data Evaluation in Enzymatic Measurements
14 Statistical Evaluation of Enzymatic Measurements Data; Presentations

Recomended or Required Reading

Textbook(s): Eisenthal, R. & J. Danson, M., Enzyme Assays: A Practical Approach, Oxford University Press, New York, 2002.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Home work

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PAR PARTICIPATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT)


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry
e-mail: leman.tarhan@deu.edu.tr

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 10 10
Preparation for final exam 1 20 20
Preparing assignments 2 15 30
Preparing presentations 2 15 30
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 172

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.15555555
LO.255555555
LO.35555555
LO.45555555
LO.555555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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