COURSE UNIT TITLE

: ENZYMOLOGY FOR RECOMBINANT DNA TECHNOLOGY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 6113 ENZYMOLOGY FOR RECOMBINANT DNA TECHNOLOGY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

M.Sc. in Biochemistry
Chemistry
Ph.D in Biochemistry

Course Objective

Within the context of this course, it is aimed to investigate in detail the enzymes used for recombinant production in medicine and industry.

Learning Outcomes of the Course Unit

1   Evaluate the structure and functions of enzyme and nucleic acids,
2   Know the used enzymes for recombinant DNA technology,
3   Understand the types and mechanisms of the recombinant DNA technology enzymes,
4   Gain knowledge about functioncs of DNA and RNA polymerase.
5   Gain knowledge about marker/reporter enzymes including ß-galactosidase, ß-lactamase, chloramphenycol acetyltransferases and luciferases.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Enzymes and Nucleic Acids-1 1.1. Structure and functions of enzymes
2 Enzymes and Nucleic Acids-2 2.1. Structure and functions of nucleic acids
3 DNA ve RNA Ligases 3.1. General properties 3.2. Escherichia coli DNA ligase 3.3. T4 DNA ligase 3.4. T4 RNA ligase
4 Nucleases 4.1. DNA endonuclease 4.2. DNA/RNA endonuclease: Staphylcoccal nükleaz 4.3. RNA endonuclease-Ribonuclease A/H/T1 4.4. Single strand spesific endonucleases
5 Exonucleases 5.1. 3'-5'- exonuclease: E.coli exonuclease I 5.2. General exonuclease: Nuklease Bal31
6 Restriction endonucleases 6.1. General properties 6.2. Type II restriction endonuclease 6.3. Type II restriction endonuclease: EcoR1 6.4. Type II restriction endonuclease: Fok1 6.5. Type I restriction endonuclease 6.6. Type III restriction endonuclease
7 Methyl Transferases 7.1. General properties 7.2. Type II Methylases: EcoR1 methyltransferase 7.3. Non-Type II Methylases:Dam ve Dcm methyltransferases
8 Midterm
9 Phosphatases and Polynucleotid Kinase 9.1. Bacterial alkaline phosphatases 9.2.Intestinal alkaline phosphatases 9.3. Polynucleotid Kinase: T4 polynucleotid kinase
10 DNA Polymerases 1 10.1. General properties 10.2. DNA-targeted DNA polymerases
11 DNA Polymerases 2 11.1. General properties of RNA- targeted DNA polymerases (reverse transcriptase) 11.2. AMV reverse transcriptase 11.3. MoLV reverse transcriptase 11.4. Terminal deoxynucleotidyl transferase
12 RNA Polymerases 12.1. General properties of DNA-targeted RNA polymerases 12.2. T7 RNA polymerase 12.3. SP6 RNA polymerase 12.4. T3 RNA polymerase 12.5. DNA-independent RNA polymerases
13 Marker/reporter enzymes 13.1. ß-galacosidase 13.2. ß-lactamase 13.3. Chloramphenycol acetyltransferases 13.4. Lusiferazlar
14 Presentation

Recomended or Required Reading

Textbook(s): Eun, H-M., 1996, Enzymology Primer for Recombinant DNA Technology, Academic Press: 683 p.
Supplementary Book(s): Wong, D.W.S., 2006, The ABCs of Gene Cloning, Springer: 229 p.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Interactive narrative
3. Question-answer technique
4. Home work

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 ASG ASSIGNMENT
2 MTE MIDTERM EXAM
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE ASG * 0.30 + MTE * 0.30 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) ASG * 0.30 + MTE * 0.30 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, active participation to the lecture, homework.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

hulya.kayali@deu.edu.tr

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 20 20
Preparation for final exam 1 30 30
Preparing assignments 2 20 40
Preparing presentations 2 15 30
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 202

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.15555555
LO.255555555
LO.35555555
LO.45555555
LO.555555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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