COURSE UNIT TITLE

: AFFINITY CHROMATOGRAPHY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 6112 AFFINITY CHROMATOGRAPHY ELECTIVE 3 0 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

M.Sc. in Biochemistry
Chemistry
Ph.D in Biochemistry

Course Objective

The aim of this lecture is to introduce advanced techniques used in izolation and purification of biomolecules.

Learning Outcomes of the Course Unit

1   To be able to understand the advanced techniques used in izolation and purification of biomolecules.
2   To be able to realize the choice of afine ligand, biospecific affinity chromatography, cell affinity chromatography, dye-Ligand chromatography, metal chelae affinity chromatography and hydrophobic interaction chromatography and explain difference between techniques.
3   To be able to explain the chemical modification of supports and immobilization of afine ligands: polysaccharides, polyacrylamides, porous glass, general reactions for coupling ligands, spacers.
4   To be able to chose the affine ligand and determine the conditions of adsorption and elution step.
5   To be able to develop the analytical abilities and ability to present and criticise arguments.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introductory remarks, History of affinity chromatography
2 General properties of the solid matrix support, Biopolymers, Synthetic polymers, Inorganic supports
3 Chemical modification of supports and immobilization of afine ligands: Polysaccharides, Polyacrylamides, Porous glass, General reactions for coupling ligands, Spacers, Blocking of unreacted groups
4 Coenzymes, Lectins, Polynucleic acids, Special variants, General consideration of the Adsorbtion and Elution step: Steric conditions, Ligand concentration, Nonspecific interaction
5 Enzymes and effectors, Immunoaffinity chromatography and Immunoassays
6 Nucleic acids: Poly(A)-affinity chromatography, Poly(A)-agaroses as affinity supports, Nucleic acid dye chromatography
7 Affinity chromatography of glycoproteins, viruses and cells, Integral membrane proteins-Amphiphatic nature, solubilization, and peculiarities in their purification by affinity methods
8 Midterm exam
9 Structure, properties, and immobilization of cibacron blue F3GA, Mechanism of dye-protein interaction, Purification of enzymes and blood proteins, Other dyes
10 Charge transfer adsorption chromatography
11 Hydrophobic interaction chromatography: Effects of neutral salts, Influence of electrostatic charges of the adsorbent material
12 Affinity partition, Affinity electrophoresis
13 High performance liquid affinity chromatography
14 Presentation of homework

Recomended or Required Reading

1. Mohr P., Pommerening K., Affinity Chromatography, Practical and Theoretical Aspects, Marcel Dekker, Inc. New York and Basel, 1986.
2. Bailon P., Ehrlich G.K., Fung W.J., Berthold W., Affinity Chromatography: Methods and Protocols, ISBN: 978-0-89603-694-9.

Planned Learning Activities and Teaching Methods

The course is taught in a lecture, class presentation and discussion format. All class members are expected to attend and both the lecture and seminar hours and take part in the discussion sessions. Besides the taught lecture, group presentations are to be prepared by the groups assigned for that week and presented to open a discussion session.

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.20 + ASG * 0.40 + FIN * 0.40
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.20 + ASG * 0.40 + RST * 0.40


Further Notes About Assessment Methods

None

Assessment Criteria

The students performance will be evaluated by the questions that will be asked in midterm (20%) and final exams (40%) and homework (40%).

Language of Instruction

English

Course Policies and Rules

Attendance to at least 70% for the lectures is an essential requirement of this course and is the responsibility of the student. It is necessary that attendance to the lecture and homework delivery must be on time. Any unethical behaviour that occurs either in presentations or in exams will be dealt with as outlined in school policy. You can find the undergraduate policy at http://web.deu.edu.tr/fen

Contact Details for the Lecturer(s)

Dokuz Eylul University, Faculty of Science, Department of Chemistry
e-mail: nalan.tuzmen@deu.edu.tr

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparations before/after weekly lectures 13 3 39
Preparation for midterm exam 1 20 20
Preparation for final exam 1 30 30
Preparing presentations 2 35 70
Final 1 2 2
Midterm 1 2 2
TOTAL WORKLOAD (hours) 202

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.155554555554
LO.255554555554
LO.355554555554
LO.455554555554
LO.555555555555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2015 DEÜ. BİD

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