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Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS MBG 5011 LABORATORY TECHNIQUES IN MOLECULAR BIOLOGY ELECTIVE 2 2 0 7

Offered By

Molecular Biology and Genetics Master Programme

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

ASISTANT PROFESSOR YAVUZ OKTAY

Offered to

Molecular Biology and Genetics Master Programme

Course Objective

To introduce the most common laboratory techniques used in the field of genomics.

Learning Outcomes of the Course Unit

1   to have a solid understanding of the basic laboratory methods used in genomics 2   to describe the in silico and wet-lab methods used to predict gene function 3   to apply data integration methods to various kinds of biological data 4   to discuss different genome editing technologies pros and cons 5   To describe the methods used for in vivo, ex vivo and in vivo genome editing 6   To explain the use of proteomic-based methods in functional genomics applications 7   To be able to perform a literature search and present findings through oral and written means.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description 1 Overview of Laboratory Techniques Used in Genomics 2 High-Resolution Analysis of Genomic Copy-Number Changes 3 Identification of Polymorphic Markers for Genetic Mapping 4 Genotyping and LOH Analysis Using SNP Arrays 5 Genetic Mapping of Complex Traits 6 Real-time Quantitative RT-PCR for mRNA profiling 7 Applications of CRISPR/Cas9 in Basic Research 8 Assessment of Gene Expression in Mammalian Cells 9 MIDTERM 10 High-throughput Investigation of Protein-Protein Interactions 11 Use of Genetically Engineered Mice to Elucidate Gene Function 12 Delivery Systems for Gene Transfer 13 Gene Therapy Strategies 14 Introduction to Proteomics Technologies 15 Assignment Presentations 16 Final Exam

Recomended or Required Reading

Textbook(s)/References/Materials: Textbook(s): Genomics: Essential Methods, Edited by Mike Starkey and Ramnath Elaswarapu, Wiley Press, 2010 ISBN: 9780470711576 Supplementary Book(s): Gene Function Analysis (Methods in Molecular Biology) 2nd edition, Springer, 2014 ISBN: 1627037209

Planned Learning Activities and Teaching Methods

Theoretical lecture with PowerPoint, literature review/discussion, and assignment presentations by students

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA 1 AS Midterm Exam 2 FN Final Exam 3 BNS Final Course Grade roundAS * 0.50+FN*0.50

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

English

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Yavuz.oktay@deu.edu.tr Phone: 412 6555

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours) Lectures 14 3 42 Tutorials 15 1 15 Preparations before/after weekly lectures 15 3 45 Preparation for midterm exam 1 25 25 Preparation for final exam 1 35 35 Preparation for quiz etc. 10 1 10 Preparing assignments 1 10 10 Final 1 2 2 Midterm 1 2 2 Quiz etc. 2 0,5 1 TOTAL WORKLOAD (hours) 187

Contribution of Learning Outcomes to Programme Outcomes

PO/LO PO.1 PO.2 PO.3 PO.4 PO.5 PO.6 PO.7 PO.8 LO.1 4 LO.2 2 LO.3 4 LO.4 2 LO.5 2 LO.6 3 LO.7 3