COURSE UNIT TITLE

: PROTEIN PURIFICATION METHODS

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
KIM 5067 PROTEIN PURIFICATION METHODS ELECTIVE 3 0 0 7

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR LEMAN TARHAN

Offered to

Chemistry
Chemistry

Course Objective

It is obligatory that proteins which are one of the macromolecules playing important roles in the life of alives are purified for the applications in many fields such as medical, nutrient and pharmacology. Methods, techniques and strategies utilised for the purification of proteins will be discussed within the context of this course.

Learning Outcomes of the Course Unit

1   Have a knowledge about protein purification methods,
2   Have knowledge about structure of proteins and amino acids so determine which method is proper for purification of proteins,
3   Interpret the possible problems during the purification process,
4   Explain the application fields of protein purification,
5   Examine high throughput screening techniques in protein purification.

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Introduction to Protein Purification
2 Gel Filtration: Size Exclusion Chromatography, Ion Exchange Chromatography
3 High Resolution Reversed-Phase Chromatography
4 Hydrophobic Interaction Chromatography
5 Immobilized Metal Ion Chromatography
6 Affinity Chromatography
7 Other Separation Methods and Related Techniques: Membran Separations
8 Midterm Exam
9 Other Separation Methods and Related Techniques 2
10 Gel Electrophoresis, Conventional Isoelectric Focusing
11 Two Dimensional Electrophoresis in Proteomics
12 Protein Elution and Blotting Techniques, Capillary Electrophoresis
13 Separation Method Optimization: High Throughput Screening Techniques in Protein Purification
14 Homework and Presentations

Recomended or Required Reading

Textbook(s): Janson J. C., Protein Purification: Principles, High Resolution Methods, and Applications, Wiley-VCH, 1998.

Planned Learning Activities and Teaching Methods

1. Presentation
2. Question-answer technique
3. Homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 PRS PRESENTATION
4 FIN FINAL EXAM
5 FCG FINAL COURSE GRADE MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + FIN * 0.40
6 RST RESIT
7 FCGR FINAL COURSE GRADE (RESIT) MTE* 0.30 + ASG * 0.20 + PRS * 0.10 + RST * 0.40

Further Notes About Assessment Methods

If the instructor needs to add some explanation or further note, this column can be selected from the DEBIS menu.

Assessment Criteria

Meaningful learning of the basic concepts given in presentations, association of concepts with each other, establishing the cause-result relationships and making comments by using the information available for problems and evaluating idea generation are carried out with mid-term and final exams, homework and presentations.

Language of Instruction

Turkish

Course Policies and Rules

Optional, if the instructor needs to add some explanation or further note, this column can be selected from the DEBIS menu.

Contact Details for the Lecturer(s)

leman.tarhan@deu.edu.tr

Office Hours

Wednesday 13.30-15.30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 13 3 39
Preparing Homework and Presentations 1 26 26
Preparation before/after weekly lectures 13 3 39
Preparation for Final Exam 1 30 30
Preparation for Mid-term Exam 1 25 25
Final 1 2 2
Mid-term 1 2 2
TOTAL WORKLOAD (hours) 163

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.15555555
LO.255555555
LO.35555555
LO.45555555
LO.555555555