COURSE UNIT TITLE

: PHYSICAL CELL SEPARATION TECHNIQUES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
BYM 0612 PHYSICAL CELL SEPARATION TECHNIQUES ELECTIVE 2 0 0 9

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

PROFESSOR ISMET GÜRHAN DELILOĞLU

Offered to

Industrial Ph.D. Program In Advanced Biomedical Technologies

Course Objective

Providing information about physical separation techniques of animal cells.

Learning Outcomes of the Course Unit

1   Understanding importance of the monotype cell culture population isolation from heterogen cultures.
2   Understanding cell separation according to the membrain markers
3   Understanding cell separation according to the physical characteristics of the cells.
4   Understanding the cell separation methods according to the growth characteristics

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 General information and basic principles
2 Cell surface markers
3 Stem Cells
4 Immunomagnetic cell seperation techniques
5 Flow cytometry
6 Designing selective surfaces and Article evaluation
7 Midterm exam
8 Density gradient cell seperation techniques
9 Selective santrifugation
10 Actual applications
11 Selective enzymatic applications
12 Article evaluation
13 General revision
14 General revision

Recomended or Required Reading

1. Cell of Cells: The Global Race to Capture and Control the Stem Cell (Hardcover) by Cynthia Fox (Author), 2007, W.W. Worton&Company Inc. 2. Culture of Animal Cells: A manual of Basic Techniques, by Freshney, I.A., 2005, Wiley-Liss, Inc., New York. 3. Embryonic Stem Cells: A Practical Approach (Practical Approach Series)(Paperback) by Elena Notarianni (Editor), 4.Martin J. Evans (Editor), 2006, Oxford University Press. 4. Handbook of Industrial Cell Culture: Mammalian, Microbial, and Plant Cells (Hardcover) by Victor A. Vinci (Editor), Sarad R. Parekh (Editor), 2003, Humana Press Inc. 5. Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology) (Hardcover) by Darwin J. Prockop (Editor), Donald G. Phinney (Editor), Bruce A. Bunnell (Editor),2008, Humana Press. 6. Related journals for recent developments.

Planned Learning Activities and Teaching Methods

homework

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 ASG ASSIGNMENT
3 FIN FINAL EXAM
4 FCG FINAL COURSE GRADE MTE * 0.20 + ASG * 0.20 + FIN * 0.60
5 RST RESIT
6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.20 + ASG * 0.20 + RST * 0.60

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

Prof. Dr. S. Ismet Deliloğlu Gürhan

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 12 3 36
Preparations before/after weekly lectures 12 7 84
Preparation for midterm exam 1 40 40
Preparation for final exam 1 50 50
Preparing assignments 1 20 20
Midterm 1 2 2
Final 1 2 2
TOTAL WORKLOAD (hours) 234

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10
LO.13531
LO.25
LO.32332
LO.44