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Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS SBE 0512 STEM CELL IMAGING TECHNIQUES ELECTIVE 2 2 0 8

Offered By

Graduate School of Natural and Applied Sciences

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR REMZIYE DEVECI

Offered to

Industrial Ph.D. Program In Advanced Biomedical Technologies

Course Objective

The purpose of this course is to introduce devices based on stem cell imaging purpose, to inform students about their working principles and to obtain experience gain about topic with its application studies.

Learning Outcomes of the Course Unit

1   To explain and apply the usage of different microscopy techniques for imaging stem cell 2   To discuss the advantages and disadvantages of microscopy types and methods which are used in alive investigations. 3   To understand and apply the imaging techniques which are used for characterization of differentiated stem cell 4   To explain the types, features, application areas and application and usage of markers which are used in stem cell imaging

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description 1 Some devices used in stem cell imaging and working principles (Microscopetypes, flow cytometry, laser scannig cytometry, vb.) Introduction of some devices used in stem cell imaging, explanation and investigation of their working principles 2 Visualization stem cells I: Light microscopy techniques To examine the implementation of appropriate methods for light microscope and visualization of stem cells 3 Visualization stem cells II: Fluorescence and Confocal Microscopy techniques To implement appropriate methods for fluorescence microscopy and visualization of stem cells 4 Visualization stem cells II: SEM preparation techniques To implement appropriate methods for stem cell investigation in SEM and discuss the methods 5 Visualization stem cells IV: TEM preparation techniquesI: Tissuepreparation techniques To implement and to examine appropriate methods for stem cell in tissue structure investigation in TEM 6 Visualization stem cells IV: TEM preparation techniquesII: Preparation techniques of isolated cells, cell aggregates and cell culture To implement and to examine appropriate methods for stem cell in culture in TEM 7 To invest alive stem cells in vitro and in vivo conditions To discuss the advantages and disadvantages of microscopy types and methods which are used in alive investigations 8 Midterm Midterm 9 Types, features, application areas and application and usage of markers used in stem cell imaging Selection and application of appropriate markers for types of stem cell 10 Nanotechnologyin stem cell imaging Inverstigation and discussion of the nanomaterials and application methods used in stem cell imaging 11 Visualization of cancer stem cell Augmentation cancer stem cells in culture in investigation in microscope 12 Visualization implamant of stem cell for therapy Investigation and discussion of photographs from literature which shows different techniques implants 13 Differentiated stem cell imaging techniques used for the characterization I: Histology, Histochemistry, Immunohistochemistry, Immunofluorescence, Cytology, Cytochemistry, immunocytochemistry, Immungold (GFP) Realization of histochemical pigmentation for bone, cartilage of fat cells which differentiated from mesenchymal stem cells and characterization of differentiation (For osteogenic differentiation alizarin red, alcian blue for chondrogenic differentiation, oil red O for adipogenic differentiation) 14 Visualization techniques for characterization of differentiation stem cell II (FISH, chromatin imaging, nanosignalling, cell sorting, immunophenotype, genetic tagging, radioimmunoassay, flow cytometry antibodies and the use of marker) Visualization of niches For chondrogenic differentiation with the use of specific antibodies to collagen II and characterization of differentiation and cytological investigation characterization in TEM Hematopoietic etc.. Visualization of stem cell niches

Recomended or Required Reading

Related studies

Planned Learning Activities and Teaching Methods

Project and exams

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA 1 MTE MIDTERM EXAM 2 ASG ASSIGNMENT 3 FIN FINAL EXAM 4 FCG FINAL COURSE GRADE MTE * 0.20 + ASG * 0.20 + FIN * 0.60 5 RST RESIT 6 FCGR FINAL COURSE GRADE (RESIT) MTE * 0.20 + ASG * 0.20 + RST * 0.60

Further Notes About Assessment Methods

None

Assessment Criteria

To be announced.

Language of Instruction

Turkish

Course Policies and Rules

To be announced.

Contact Details for the Lecturer(s)

To be announced.

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours) Lectures 14 2 28 Tutorials 14 2 28 Preparations before/after weekly lectures 14 4 56 Reading 14 2 28 Preparing presentations 1 9 9 Preparation for final exam 1 28 28 Preparation for final exam 1 28 28 Final 1 2 2 Midterm 1 2 2 TOTAL WORKLOAD (hours) 209

Contribution of Learning Outcomes to Programme Outcomes

PO/LO PO.1 PO.2 PO.3 PO.4 PO.5 PO.6 PO.7 PO.8 PO.9 PO.10 LO.1 4 LO.2 4 LO.3 5 4 5 LO.4 5