COURSE UNIT TITLE

: LABORATORY ETECHNICS FOR ELECTRON MICROSCOPY

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
HIS 5024 LABORATORY ETECHNICS FOR ELECTRON MICROSCOPY ELECTIVE 1 4 0 9

Offered By

Histology and Embryology

Level of Course Unit

Second Cycle Programmes (Master's Degree)

Course Coordinator

PROFESSOR IŞIL TEKMEN

Offered to

Biochemistry
Histology and Embryology
Medical Parasitology

Course Objective

To provide learning methods of tissue preparation, sectioning and contrasting for electron microscope.

Learning Outcomes of the Course Unit

1   Define types of fixatives and to prepare fixatives for electron microscopy,
2   Identify and apply methods for electron microscopic tissue preparation
3   Able to using ultramicrotome
4   Contrasting method of thin sections
5   Able to make presentations according to the related topics

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Fixation, preparation of Fixatives and Buffers I
2 Fixation, preparation of Fixatives and Buffers II
3 Fixation, preparation of Fixatives and Buffers III
4 Procedures of tissue preparing I
5 Procedures of tissue preparing II
6 Procedures of tissue preparing III
7 Procedures of tissue preparing IV
8 Trimming
9 Glass knife making
10 Semi-thin sectioning with ultramicrotome I
11 Semi-thin sectioning with ultramicrotome II
12 Thin sectioning with ultramicrotom I
13 Thin sectioning with ultramicrotom II
14 Contrasting

Recomended or Required Reading

Textbook(s):
1. Histology: A Text&Atlas (With_Correlated_Cell_and_Molecular_Biology__6th_Edition) Ross2011
2. Color Textbook of . Histology leslie P. Gartner , James L. Hiatt 2006
3-Histological & Histochemical Methods Theory & Practice J:A: Kiernan Arnold London 1999.
References:
Materials: Olympus BH2 light microscopy, Leica ULTRACUT, Carl Zeiss EFTEM electron microscope

Planned Learning Activities and Teaching Methods

Lectures and Application

Assessment Methods

SORTING NUMBER SHORT CODE LONG CODE FORMULA
1 MTE MIDTERM EXAM
2 FIN FINAL EXAM
3 FCG FINAL COURSE GRADE MTE * 0.40 + FIN* 0.60
4 RST RESIT
5 FCGR FINAL COURSE GRADE MTE * 0.40 + RST* 0.60

Further Notes About Assessment Methods

None

Assessment Criteria

Optional, if the instructor needs to add some explanation or further note, this column can be selected from the DEBIS menu.

Language of Instruction

Turkish

Course Policies and Rules

Optional, if the instructor needs to add some explanation or further note, this column can be selected from the DEBIS menu.

Contact Details for the Lecturer(s)

412 45 54 - isil.tekmen@deu.edu.tr

Office Hours

To be announced.

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 1 15
Tutorials 15 4 60
Preparations before/after weekly lectures 15 3 45
Preparation for final exam 1 10 10
Preparing presentations 15 2 30
Lab Exam Preparation 15 4 60
Final 1 5 5
TOTAL WORKLOAD (hours) 225

Contribution of Learning Outcomes to Programme Outcomes

PO/LOPO.1PO.2PO.3PO.4PO.5PO.6PO.7PO.8PO.9PO.10PO.11
LO.155
LO.2544455
LO.355
LO.4535
LO.5555

CONTACT INFORMATION
Dokuz Eylül Üniversitesi Cumhuriyet Bulvarı No: 144 35210 Alsancak / İZMİR
Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

Copyright 2012 DEÜ. BİD

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