COURSE UNIT TITLE

: BASIC LABORATORY PRINCIPLES AND METHODS I

DEGREE PROGRAMMES

Description of Individual Course Units

Course Unit Code Course Unit Title Type Of Course D U L ECTS
ERS 6007 BASIC LABORATORY PRINCIPLES AND METHODS I ELECTIVE 1 2 0 10

Offered By

Biochemistry

Level of Course Unit

Third Cycle Programmes (Doctorate Degree)

Course Coordinator

ASSOCIATE PROFESSOR ZÜBEYDE ERBAYRAKTAR

Offered to

Biochemistry

Course Objective

The purpose of this course is to teach basic laboratory principles and general laboratory procedures and also to introduce separation techniques. The course consists of theory and practice sections. This course is given as a mini project. In theoretical section of the course, the basic principles and procedures regarding the method are given.
In the implementation stage of the course, the supernatants are obtained from rat tissues (brain, liver, heart, kidney, lung) by weighing, homogenization and centrifugation. The supernatants obtained are used in all methods to learn throughout the semester. In these supernatants primarily the protein concentrations are determined using spesific protein methods like Biuret, Modified Lowry and Bradford.
Then, Glutathione-S-transferase and MDA levels are measured by using a spectrophotometer, spectrofluorimeter and Liquid Chromatography (HPLC) method, respectively. The molecular weights "MW" of unknown protein bands are calculated by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).The is Students make presentations related with their results in the end of the semester.

Learning Outcomes of the Course Unit

1   Be acquired the knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
2   Be grasped the theoretical knowledge about the basic biochemistry and basic chemistry which are the main areas of biochemistry.
3   Have the knowledge about the principles and methods of basic laboratory equipments.
4   Be able to critically analyze the basic concepts of basic laboratory methods (eg, such as differences in methods of protein determination)
5   Synthesis: Be able to discuss the learned methods by considering different ways and be able to adapt to new situations (Example: to be able to discuss why different tissues contain different proteins)
6   Be able to present by explicating the results of one of the applied methods.
7   Be acquired the basic laboratory skills ( for example: to weigh, buffer preparation, pH measurement, to make homogenize, to make centrifuge)
8   Be acquired the application skills of the general laboratory prosedures

Mode of Delivery

Face -to- Face

Prerequisites and Co-requisites

None

Recomended Optional Programme Components

None

Course Contents

Week Subject Description
1 Laboratory Safety, Principles of the laboratory working, General Laboratory Equipment, Laboratory Preparation, Principles of Weighing, SI Unit, Solution Preparation (Concentration Calculations), Measurement of pH and buffer preparation, Homogenization techniques, Centrifugation, the basic principles and types, Spectrophotometry; Glutathione-S-transferase measurement with spectrophotometer
2 Protein content measurement using a spectrophotometer, Protein determination by Biüret, Lowry, Bradford and BCA Method, practice, Spectrofluorimeter with the measurement of MDA, Types and Applications of Chromatography, (HPLC) method, Types and Applications of Electrophoresis, Preparation of Electrophoresis Buffer, Gel cast, execution and paint, Quantification of protein bands

Recomended or Required Reading

Textbook(s):
Tietz: Klinik Kimyada Temel Ilkeler Beşinci Baskı. (Editörler: Burtis & Ashwood) (Çeviri Editörü: Diler Aslan).PALMY yayıncılık, pp.352-387.
http://www.ruf.rice.edu/~bioslabs/methods/protein/protein.html
http://www.ruf.rice.edu/~bioslabs/tools/graphs/dataplot.html

Planned Learning Activities and Teaching Methods

This course consists of theory and practical sections. This course is given as a mini project. In theoretical section of the course, the basic principles and procedures regarding the method are given.

Assessment Methods

Successful / Unsuccessful

Further Notes About Assessment Methods

If needed, other assessment methods can be added to the table given below.

Assessment Criteria

Optional, if the instructor needs to add some explanation or further note, this column canbe selected from the DEBIS menu.
Comprehension, learning, analysis, comparison, applications, interactive participation

Language of Instruction

English

Course Policies and Rules

Optional, if the instructor needs to add some explanation or further note, this column can be selected from the DEBIS menu.

Contact Details for the Lecturer(s)

e-mail : gulgun.oktay@deu.edu.tr Telefon: 412 44 02 GSM: + 5363548774

Office Hours

Friday 12.30-13:30

Work Placement(s)

None

Workload Calculation

Activities Number Time (hours) Total Work Load (hours)
Lectures 15 1 15
Tutorials 30 1 30
Preparation for midterm exam 1 20 20
Preparing presentations 1 20 20
Preparations before/after weekly lectures 29 5 145
Preparation for final exam 1 14 14
Midterm 1 3 3
Final 1 3 3
TOTAL WORKLOAD (hours) 250

Contribution of Learning Outcomes to Programme Outcomes

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CONTACT INFORMATION
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Phone: +90(232) 412 12 12 - Fax: +90 (232) 464 81 35

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